The Milo single-cell protein expression quantitative analysis system uses a designed microfluidic single-cell immunoblotting chip (scWest). The single-cell chip can separate and collect thousands of single cells in a single run, release proteins in situ, separate them by electrophoresis according to different molecular weights, and then perform immunological detection. After collecting signals with a scanner, software analysis and processing are used to achieve single-cell multiplex protein quantitative analysis.

unicellularWB analysis can divide cells into three groups: ① single expression of Exon1, ② single expression of Exon2, and ③ simultaneous expression of Exon1 and Exon2. Traditional WB can only detect the expression of Exon1 and Exon2, but cannot classify heterogeneous cells

Milo Single Cell Solution

Milo single-cell protein expression quantitative analysis technology can achieve quantitative analysis of protein expression within individual cells, finely analyze protein molecular differences, and reveal the types and status differences between different individual cells. Detecting proteins without good flow cytometry antibodies, exploring the heterogeneity of protein isoforms, quantifying transduction efficiency, and better understanding cell phenotypes and life activities.

unicellularRNA sequencing is an important tool for stem cell differentiation, cell profiling, and tumor heterogeneity research. Single cell RNA sequencing (scRNASeq) combined with Milo single cell sequencing

Cellular protein expression analysis can fully elucidate the role of key genes.

System Characteristics

High throughput detection: A single chip can capture up to 1000 single cells for protein detection

Multiple protein analysis: A single test can detect up to 4 target proteins, and elution followed by hybridization can detect up to 12+heavy proteins

Fast speed, full process inspection~4 hours

Western antibodies and fluorescent antibodies